Plasmid-DNA Quote Form

First Name:
Last Name:
Email Address:
Company/Institution:

Plasmid-Name
Requested Amount (mg)
Total size of plasmid (kb)
Antibiotic Resistance
 		Other:
Preferred Host
 		Other:
Final Buffer
 		Other:
Final Concentration (mg/mL)
 		>0.5mg/mL(standard) Other:
Endotoxin requirement
 		Our standard specification is <100EU/mg. 
Lower amounts can be achieved but additional fees may apply.
pDNA homogeneity
 		predominantly supercoiled (standard)>85%supercoiled

Standard Specifications
Test Method Specifications
260/280 by UV Spectrophotomerty 1.8 - 2.0
Appearance by Visual Inspection clear and colorless
pDNA homogeneity by Agarose Gel Electrophoresis predominantly supercoiled
Residual Endotoxin by LAL <100 EU/mg
Genomic DNA by Agarose Gel Electrophoresis <5.0%
Identity by Agarose Gel Electrophoresis
 co-migrates with reference DNA and/or size confirmed versus supercoiled marker
Residual RNA by Agarose Gel Electrophoresis None Detected (0.2 µg loaded)
pDNA concentration by UV Spectrophotometry >0.5mg/mL

Additional tests and requirements (check all that apply) (additional fees apply)
Bioburden
 		Would you like to add the Bioburden test?
BCA Protein Assay Would you like to add the BCA test for residual protein?
Restriction digest

List each Enzyme/# framents/expected sizes:
(for example: BamHI/2/1544,3812; HindIII/1/5356)

Archive
 		Would you like us to archive your plasmid for possible future orders?
Special Dispensing
 		Please indicate if you would like your plasmid dispensed such that there is a certain volume per vial otherwise it will be shipped all in 1 vial.
Please provide the following information about your plasmid:
Are there any known stability issues with your plasmid?
 		No   Yes, please explain below:
What is the expected yield?

<3 mg/L    3 - 6 mg/L   >6mg/L
What is the expected yield based on?
 		Experience    Prediction   Complete guess
Check any special growth conditions that apply.
 		Temperature;
Media;
Other;

Special requirements and/or comments: